The MTT Assay: Utility, Limitations, Pitfalls, and Interpretation in Bulk

To prepare a 12 mM MTT stock solution, dissolve 5 mg of MTT in 1 mL PBS This stock solution is enough for 100 reactions

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mtt range charts Following reconstitution, aliquot and freeze Stock solutions are stable for up to 1 month at -20°C Warning Toxicity: Harmful & Carcinogenic

mtt stock Each vial of MTT provides sufficient reagent for 100 tests, using 10 µL of the stock solution per well 6 Once prepared, the MTT work solution can be stored