Effect of growth media on the MTT colorimetric assay in bacteria

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MTT is used to assess cell viability as a function of redox potential Actively respiring cells convert the water-soluble MTT to an insoluble purple

MTT BASED Stock No CGD-1 Store at -0 °C This kit is designed for the spectrophotometric measurement of cell growth as a function of mitochondrial activity Prior to starting the assay, 5 mgml MTT stock solution with sterile PBS should be freshly prepared Dimethyl sulfoxide is needed to

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