Efficient ultrafiltration‐based protocol to deplete extracellular

cyquant protocol   Dan cyquant protocol

A two-step warming protocol was shown to improve the cell recovery in this adherent cell model Frozen plates were removed from -135°C storage and placed

This protocol uses DMSO as a solubilizing agent for formazan to shorten the time of the assay Replace the culture medium This protocol uses DMSO as a solubilizing agent for formazan to shorten the time of the assay Replace the culture medium

rabona kick Cyquant) • Remove plate from the machine and place it on ice • Wash 2Â protocol • Defrost assay plate and add 200 μL of lysis buffer per well Assay principle: The CyQUANT Direct Cell Proliferation Assay is based on a DNA-binding dye which is cell permeable Dead cells are blocked from staining by a